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Image Search Results
Journal:
Article Title: Low levels of Na, K-ATPase and carbonic anhydrase II during choroid plexus development suggest limited involvement in early CSF secretion
doi: 10.1016/j.neulet.2008.06.068
Figure Lengend Snippet: NKAα1 immunoreactivity (A–B) was present on in the choroid plexus at all ages examined from E15 to adult. At E15 (A) only a few cells were positive (arrows) and the number of positive cells increased with age and at P9 (B) most cells were strongly immunopositive (arrows). The immunoreactivity was located exclusively on the apical membrane at all ages examined (E15, E18, P0, P9 and adult). CAII immunoreactivity (C–D) was not detected between E15 (C) and P0 (not illustrated), but was detected at P9 (D). At P9 the immunoreactivity was cytosolic and present in most of the epithelial cells (arrows). Sections were counterstained with toluidine blue. E is the embryonic day, P is the postnatal day, NKAα1 is the Na, K-ATPase α1-subunit and CAII is carbonic anhydrase II. Scale bar 50 μm.
Article Snippet: The membrane was incubated with primary antibodies against Na,
Techniques: Membrane
Journal:
Article Title: Low levels of Na, K-ATPase and carbonic anhydrase II during choroid plexus development suggest limited involvement in early CSF secretion
doi: 10.1016/j.neulet.2008.06.068
Figure Lengend Snippet: Western Blot analysis of NKAα1 and CAII during choroid plexus development in the rat. (A) The developmental profile of NKAα1 in isolated lateral ventricular choroid plexus showed an increase in band intensity with increasing age. (B) The developmental profile of CAII showed a progressive increase in band intensity with age. CAII immunoreactivity was detected at all ages from E15. The signal from blood and choroid plexus samples containing the same amount of protein were compared. A distinct band was detected in the blood sample indicating that some of the signal from the highly vascularised choroid plexus might be due to blood contamination. E is the embryonic day, P is the postnatal day, A is adult, NKAα1 is the Na, K-ATPase α-subunit, CAII is carbonic anhydrase II, A × 1.5 represents loading of 50% more protein in this lane to show that the signal was not saturated, CP is choroid plexus and Bl. is blood.
Article Snippet: The membrane was incubated with primary antibodies against Na,
Techniques: Western Blot, Isolation